Determination of Vitamin B3 Levels on Red Dragon Fruit (Hylocereus Costaricensis (F.A.C Weber) Britton & Rose) Using HPLC
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Abstract
Dragon fruit (Hylocereus costaricensis (F.A.C Weber) Britton & Rose) contains bioactive substances that are beneficial to the body, including antioxidants. This study aims to determine the levels of vitamin B3 in red dragon fruit (Hylocereus costaricensis (F.A.C Weber) Britton & Rose) using High Performance Liquid Chromatography. Red dragon fruit is taken from Kamang Magek District, Agam Regency, West Sumatra. Red dragon fruit samples (Hylocereus costaricensis (F.A.C Weber) Britton & Rose) were extracted by maceration. Then the sample extract was added with 5M NaOH and HCl and analyzed by High Performance Liquid Chromatography using methanol and phosphate buffers (10:90) as the mobile phase against UV light at a wavelength of 261 nm. The results showed that the mean level of Vitamin B3 from (Hylocereus costaricensis (F.A.C Weber) Britton & Rose) was 0.0173465 mg with a detection limit of 0.515 g /mL and a limit of quantization of 1.7190 g / mL.
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